Mouse GFRA3 ELISA Kit PicoKine®

Mfr.No.

EK2076

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.036	0.144	0.245	0.468	0.821	1.315	1.908	2.241

Description

Mouse GFRA3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gfra3 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

GDNF family receptor alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; Gfra3

Immunogen

Expression system for standard: NS0; Immunogen sequence: N29-N371

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse GFRA3

Subcellular Localization

Cell membrane. GPI-anchor.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	26	109	355	29	112	401
Standard deviation	1.25	5.78	19.88	1.48	6.38	24.86
CV (%)	4.8%	5.3%	5.6%	5.1%	5.7%	6.2%

Protein Function

Receptor for the glial cell line-derived neurotrophic factor, artemin. Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase.

Background

GDNF family receptor alpha-3 (GFR?3), also known as the artemin receptor, is a protein that in humans is encoded by the GFRA3 gene. It is mapped to 18; 18 B1. The protein encoded by this gene is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	26	27	26	25	26	0.70	2.6%
Sample 2	109	109	105	93	104	6.55	6.2%
Sample 3	355	365	388	351	364	14.35	3.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	14587
Gene Name	Gfra3
UniProt ID	O35118

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001588	EK2076	96 wells/kit	$499		Add to Cart

Mouse GFRA3 ELISA Kit PicoKine®

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