Mouse GFRA1 ELISA Kit PicoKine®

Mfr.No.

EK2070

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.039	0.130	0.242	0.507	0.824	1.311	1.776	2.094

Description

Mouse GFRA1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gfra1 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; TGF-beta-related neurotrophic factor receptor 1; Gfra1; Gdnfra; Tr1

Immunogen

Expression system for standard: NS0; Immunogen sequence: D25-S430

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse GFRA1

Subcellular Localization

Cell membrane. GPI-anchor.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	78	249	857	81	254	955
Standard deviation	3.20	11.45	45.42	4.46	17.02	71.63
CV (%)	4.1%	4.6%	5.3%	5.5%	6.7%	7.5%

Protein Function

Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.

Background

GDNF family receptor alpha-1 (GFR?1), also known as the GDNF receptor, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to 19 D2; 19 54.18 cM. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Two alteatively spliced transcript variants encoding different isoforms have been described for this gene.GDNF family receptor alpha 1 has been shown to interact with GDNF and RET proto-oncogene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	17	16	16	16	16	0.43	2.6%
Sample 2	89	96	104	99	97	5.43	5.5%
Sample 3	450	497	503	495	486	21.13	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	14585
Gene Name	Gfra1
UniProt ID	P97785
Tissue Specificity	Expressed in heart, brain, lung, liver, kidney and testis.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001582	EK2070	96 wells/kit	$499		Add to Cart

Mouse GFRA1 ELISA Kit PicoKine®

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