Mouse GDF-15 ELISA Kit PicoKine®

Mfr.No.

EK1134

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.043	0.106	0.145	0.279	0.483	0.851	1.501	2.046

Description

Mouse GDF-15 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gdf15 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth/differentiation factor 15;GDF-15;Gdf15;Sbf;

Molecular Weight

33225 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S189-A303

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse GDF-15

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	36	233	386	39	238	388
Standard deviation	1.76	10.01	23.16	2.45	14.28	24.3%
CV (%)	4.9%	4.3%	6%	6%%	6%	6.6%

Background

GDF-15 (Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by ly interfering with chemokine signaling and integrin activation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	36	32	31	30	32	2.27	7%
Sample 2	233	243	249	254	244	7.82	3.2%
Sample 3	386	396	409	421	403	13.2	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	23886
Gene Name	GDF15
UniProt ID	Q9Z0J7
Protein Name	Growth/differentiation factor 15
Tissue Specificity	Highly expressed in liver. .

Applications	ELISA
Research Area	Cardiovascular,Cell Biology,Cell Cycle,Cell Division,Cytokinesis,Cytoskeleton/ECM,Extracellular Matrix,Growth Factors/Hormones,Hypertrophy,Signal Transduction,Structures

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000783	EK1134	96 wells/kit	$499		Add to Cart

Mouse GDF-15 ELISA Kit PicoKine®

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