Mouse Galectin-7/LGALS7 ELISA Kit PicoKine®

Mfr.No.

EK1279

Sensitivity

<10 pg/ml

Detection Range

93.8 pg/ml - 6,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	93.8	187.5	375	750	13000	3000	6000
O.D.	0.084	0.128	0.178	0.253	0.444	0.658	1.511	2.327

Description

Mouse Galectin-7/LGALS7 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lgals7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Galectin-7;Gal-7;Lgals7;

Molecular Weight

15173 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-F136

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Galectin-7

Subcellular Localization

Cytoplasm . Nucleus . Secreted . May be secreted by a non-classical secretory pathway. .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	161	861	2074	152	805	2004
Standard deviation	6.6	40.46	163.84	7.44	42.66	168.33
CV (%)	4.1%	4.7%	7.9%	4.9%	5.3%	8.4%

Protein Function

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release (By similarity). .

Background

Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	161	138	155	158	153	8.91	5.8%
Sample 2	861	902	914	909	896	20.93	2.3%
Sample 3	2074	2175	2110	2264	2155	72.22	3.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16858
Gene Name	LGALS7
UniProt ID	O54974
Protein Name	Galectin-7

Applications	ELISA
Research Area	Apoptosis,Cancer,Cell Adhesion Proteins,Cell Death,Invasion/Microenvironment,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000869	EK1279	96 wells/kit	$499		Add to Cart

Mouse Galectin-7/LGALS7 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse Galectin-7/LGALS7 ELISA Kit PicoKine®

Rate it now..!!