Mouse Galectin-1 ELISA Kit PicoKine®

Mfr.No.

EK0763

Sensitivity

<5 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.033	0.111	0.179	0.301	0.574	0.890	1.500	2.085

Description

Mouse Galectin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lgals1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Galectin-1;Gal-1;14 kDa lectin;Beta-galactoside-binding lectin L-14-I;Galaptin;Lactose-binding lectin 1;Lectin galactoside-binding soluble 1;S-Lac lectin 1;Lgals1;Gbp;

Molecular Weight

14866 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-E135

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Galectin-1

Subcellular Localization

Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	343	1747	4774	318	1697	4684
Standard deviation	22.29	132.77	300.76	20.67	144.24	304.46
CV (%)	6.5%	7.6%	8.5%	6.5%	8.5%	6.5%

Protein Function

May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity. Strong inducer of T-cell apoptosis (By similarity). .

Background

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	343	316	342	346	336	12.07	3.5%
Sample 2	1747	1764	1871	1625	1751	87.26	4.9%
Sample 3	4774	4662	4642	4948	4756	121.46	2.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16852
Antigen	Galectin-1
Gene Name	LGALS1
UniProt ID	P16045
Protein Name	Galectin-1
Sequence	Contains 1 galectin domain.

Applications	ELISA
Research Area	Cell Biology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000608	EK0763	96 wells/kit	$499		Add to Cart

Mouse Galectin-1 ELISA Kit PicoKine®

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