Matrices listed below were spiked with certain level of Gal and the recovery rates were calculated by comparing the measured value to the expected amount of Gal in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
98
EDTA plasma(n=5)
87-104
96
heparin plasma(n=5)
90-102
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gal and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Gal ELISA Kit allows for the in vitro quantitative determination of Gal concentrations in serum, plasma, tissue homogenates and other biological fluids.