Mouse Fructosamine-3-kinase

Mfr.No.

E1354Mo

Sensitivity

14.36ng/L

Detection Range

30-9000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

FN 3K;FN3K;Fructosamine-3-kinase;Protein-psicosamine 3-kinase FN3K;Protein-ribulosamine 3-kinase FN3K

Molecular Weight

35032

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1354Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1243.5	52.31	4.2
Intra-Assay	2	18	1823.3	78.25	4.3
Intra-Assay	3	18	4199.8	118.34	2.8

Background

Fructosamine-3-kinase involved in protein deglycation by mediating phosphorylation of fructoselysine residues on glycated proteins, to generate fructoselysine-3 phosphate (PubMed: 11016445, PubMed: 16819943). Fructoselysine-3 phosphate adducts are unstable and decompose under physiological conditions (By similarity). Involved in intracellular deglycation in erythrocytes and pancreatic islets (PubMed: 16819943, PubMed: 20009024). Involved in the response to oxidative stress by mediating deglycation of NFE2L2/NRF2, glycation impairing NFE2L2/NRF2 function (PubMed: 31398338). Also able to phosphorylate psicosamines and ribulosamines (By similarity).

Protocol

These standard curves of E1354Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.155	2.011	1.971
	1.868
2400ng/L	1.06	1.065	1.025
	1.069
1200ng/L	0.519	0.492	0.451
	0.464
600ng/L	0.191	0.194	0.153
	0.196
300ng/L	0.168	0.146	0.106
	0.124
0ng/L	0.032	0.04	0
	0.049

Gene ID	63828
UniProt ID	Q9ER35

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109218	E1354Mo	48T	$320		Add to Cart
BHE12109218	E1354Mo	96T	$458		Add to Cart

Mouse Fructosamine-3-kinase - FN3K ELISA Kit

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