Mouse Free Thyroxine,FT4 ELISA Kit

Mouse Free Thyroxine,FT4 ELISA Kit

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Mfr.No.

CSB-E05080m

Alternative Names

Free Thyroxine,FT4

Reactivity

Mouse

Detection Range

4 pmol/L-64 pmol/L

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of hamster IFNG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	92	88-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of hamster IFNG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	84
	Range %	80-88
1:4	Average %	89
	Range %	84-93
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
120	2.584	2.727	2.656	2.512
60	2.028	2.106	2.067	1.923
30	1.265	1.334	1.300	1.156
15	0.714	0.755	0.735	0.591
7.5	0.371	0.347	0.359	0.215
3.75	0.293	0.309	0.301	0.157
1.87	0.207	0.217	0.212	0.068
0	0.146	0.142	0.144

Species

Mus musculus

Sensitivity

1.08 pmol/L

Limit Of Detection

450 nm

Antigen	Free Thyroxine,FT4

Applications

ELISA

Research Area

FT4

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505749	CSB-E05080m	96T	$395	Add to Cart
BHE10505749	CSB-E05080m	5x96T	$1896	Add to Cart
BHE10505749	CSB-E05080m	10x96T	$3752.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.