Mouse Fractalkine,FK ELISA Kit

Mouse Fractalkine,FK ELISA Kit

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Mfr.No.

CSB-E04559m

Sensitivity

31.25 pg/mL

Detection Range

125 pg/mL-8000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

chemokine (C-X3-C motif) ligand 1, ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin, small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin), small inducible cytokine subfamily D (

Recovery

The recovery of rat TRIM72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TRIM72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	99
	Range %	92-110
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.814	2.989	2.902	2.817
600	2.366	2.441	2.404	2.319
300	1.559	1.457	1.508	1.423
150	0.753	0.697	0.725	0.640
75	0.469	0.442	0.456	0.371
37.5	0.243	0.235	0.239	0.154
18.75	0.149	0.139	0.144	0.059
0	0.088	0.082	0.085	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O35188

Applications	ELISA
Research Area	CX3CL1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.