Mouse FOLR1/Alpha Fr ELISA Kit PicoKine®

Mfr.No.

EK1243

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	46.9	94	188	375	750	1500	3000
O.D.	0.032	0.087	0.134	0.235	0.409	0.722	1.329	2.210

Description

Mouse FOLR1/Alpha Fr ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Folr1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Folate receptor alpha;FR-alpha;Folate receptor 1;Folate-binding protein 1;Folr1;Fbp1, Folbp1;

Molecular Weight

29449 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T25-S232

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse FOLR1

Subcellular Localization

Cell membrane; Lipid-anchor, GPI-anchor. Secreted . Cytoplasmic vesicle . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Apical cell membrane. Endocytosed into cytoplasmic vesicles and then recycled to the cell membrane (By similarity). Detected at proximal tubule apical membranes. .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	102	398	1282	100	358	1344
Standard deviation	5.4%1	21.49	67.94	5.3%	20.04	73.92
CV (%)	5.5%	5.4%	5.3%	5.9%	5.6%	5.5%

Protein Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation. Required for renal folate reabsorption. .

Background

Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein (FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	102	96	88	100	96	5.36	5.5%
Sample 2	398	411	396	401	401	5.76	1.4%
Sample 3	1282	1443	1389	1334	1362	60.15	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	14275
Antigen	FOLR1/Alpha
Gene Name	FOLR1
UniProt ID	P35846
Protein Name	Folate receptor alpha
Tissue Specificity	Detected in kidney proximal tubules (at protein level). .

Applications	ELISA
Research Area	Cell Type Markers,Tags & Cell Markers,Tumor Associated

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000848	EK1243	96 wells/kit	$499		Add to Cart

Mouse FOLR1/Alpha Fr ELISA Kit PicoKine®

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