Mouse follicle-stimulating hormone,FSH ELISA Kit

Mouse follicle-stimulating hormone,FSH ELISA Kit

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Mfr.No.

CSB-E06871m

Sensitivity

2.5 mIU/ml

Detection Range

4 mIU/ml-140 mIU/ml

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Recovery

The recovery of rabbit MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-103
EDTA plasma (n=4)	92	87-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	89
	Range %	85-95
1:20	Average %	96
	Range %	90-105
1:40	Average %	93
	Range %	85-99
1:80	Average %	94
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.506	2.531	2.519	2.419
50	2.048	2.005	2.027	1.927
25	1.674	1.691	1.683	1.583
12.5	1.184	1.207	1.196	1.096
6.25	0.882	0.879	0.881	0.781
3.12	0.554	0.543	0.549	0.449
1.56	0.306	0.314	0.310	0.210
0	0.102	0.097	0.100

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q60687

Applications	ELISA
Research Area	FSH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Inhibin a-Subunit N-terminus Interacts With Activin Type IB receptor To Disrupt Activin Signaling Zhu J et al,The Journal of Biological Chemistry,2012 Mar 9;287(11):8060-8070 Development and Characterization of Cell-Specific Androgen Receptor Knockout Mice /,The Nuclear Receptor Superfamily: Methods and Protocols,219-248. Bu Shen Tiao Chong recipe restores diminished ovary reserve through the BDNF pathway. Xia T.et al,J Assist Reprod Genet.,33(6):795-805 Bisphenol A Exposure during Pregnancy Alters the Mortality and Levels of Reproductive Hormones and Genes in Offspring Mice Ma S.et al,Biomed Res Int.,Mar 14. Genotoxic and Hypogonadism Effect of Triclosan Treatment and the Mitigating Effect of Vitamin E in Male Albino Mice Nahed A Hussien.et al,Journal of Basic and Clinical Pharmacy,/ Reintroducing testosterone in the db/db mouse partially restores normal glucose metabolism and insulin resistance in a leptin-independent manner Koichi Yabiku.et al,BMC Endocrine Disorders,June 13 Lycopene reduces in utero bisphenol A exposure-induced mortality, benefits hormones, and development of reproductive organs in offspring mice Shuang Ma.et al,Environmental Science and Pollution Research,June 8 Determine the Role of FSH Receptor Binding Inhibitor in Regulating Ovarian Follicles Development and Expression of FSHR and ERα in Mice Luju Lai.et al,BioMed Research International,July 9 Nasal delivery of nerve growth factor rescue hypogonadism by up-regulating GnRH and testosterone in aging male mice Jiao Luo .et al,EBioMedicine,August 18

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BHE10505738	CSB-E06871m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.