Mouse Filaggrin - FLG ELISA Kit

Mfr.No.

E1639Mo

Sensitivity

0.58ng/L

Detection Range

1-400ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Filaggrin (also known as FLG) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AW107830;fi;Filaggrin;FLG;Fragment;ft

Molecular Weight

35678

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1639Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.98	1.37	7.59
Intra-Assay	2	18	89.11	5.16	5.78
Intra-Assay	3	18	62.26	3.27	5.25

Background

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

Protocol

These standard curves of E1639Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/L	2.214	2.2	2.157
	2.187
120ng/L	1.538	1.536	1.493
	1.535
60ng/L	1.172	1.161	1.117
	1.149
30ng/L	0.733	0.69	0.647
	0.647
15ng/L	0.475	0.485	0.442
	0.496
0ng/L	0.047	0.043	0
	0.039

Gene ID	14246
UniProt ID	P11088

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109481	E1639Mo	48T	$320		Add to Cart
BHE12109481	E1639Mo	96T	$458		Add to Cart

Mouse Filaggrin - FLG ELISA Kit

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