Mouse Fibulin-1(FBLN1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL008452MO

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

fibulin 1, CTA-941F9.7, FBLN

Recovery

The recovery of pig mucin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-96
EDTA plasma (n=4)	98	95-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig mucin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	90
	Range %	87-98
1:8	Average %	92
	Range %	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q08879
Tissue Specificity	Detected in most organs (brain, heart, lung, spleen, liver and kidney). Neurons are the predominant source of production in the brain. Not expressed significantly by astrocytes or microglia.

Applications	ELISA
Research Area	FBLN1

Storage	Store at 2-8°C. Please refer to protocol.

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