Mouse FCRN/FCGRT ELISA Kit PicoKine®

Mfr.No.

EK1454

Sensitivity

<10 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	13000	3000	6000
O.D.	0.075	0.117	0.170	0.236	0.385	0.744	1.337	2.224

Description

Mouse FCRN/FCGRT ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fcgrt in cell culture supeatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

IgG receptor FcRn large subunit p51;FcRn;IgG Fc fragment receptor transporter alpha chain;Neonatal Fc receptor;Fcgrt;Fc;

Molecular Weight

40093 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-S297

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse FCRN

Subcellular Localization

Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma, Cell lysates

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	132	1019	2423	143	1117	2272
Standard deviation	4%8	71.33	118.72	7.57	99.41	134.04
CV (%)	4%	7%	4.9%	5.3%	8.9%	5.9%

Protein Function

Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the selective uptake of IgG from milk and helps newbo animals to acquire passive immunity. IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids.

Background

The neonatal Fc receptor (FcRn), also known as the Brambell receptor, is a protein that in humans is encoded by the FCGRT gene. The neonatal Fc receptor is an Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. It was first discovered in rodents as a unique receptor capable of transporting IgG from mother's milk across the epithelium of newbo rodent's gut into the newbo's bloodstream. Further studies revealed a similar receptor in humans, leading to the naming as a neonatal Fc receptor. In humans, however, it is found in the placenta to help facilitate transport of mother's IgG to the growing fetus and it has also been shown to play a role in monitoring IgG tuover. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine,TNF-?, and down-regulated by IFN-?.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	132	123	130	122	126	4.32	3.4%
Sample 2	1019	1070	915	953	989	59.65	6%
Sample 3	2423	2361	2207	2520	2377	113.7	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	14132
Gene Name	Fcgrt
UniProt ID	Q61559
Protein Name	IgG receptor FcRn large subunit p51
Tissue Specificity	Intestinal epithelium of suckling rodents. Expressed in neonatal intestine and fetal yolk sac.

Applications	ELISA
Research Area	Adaptive Immunity,Class I,Immunoglobulins,Immunology,Receptors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001077	EK1454	96 wells/kit	$750		Add to Cart

Mouse FCRN/FCGRT ELISA Kit PicoKine®

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