Mouse FCRL5 ELISA Kit PicoKine®

Mfr.No.

EK1978

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1,000
O.D.	0.121	0.544	0.689	0.950	1.381	1.720	2.028	2.179

Description

Mouse FCRL5 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse FCRL5 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Fc receptor-like protein 5; FcR-like protein 5; FcRL5; BXMAS1-like protein 2; Mbxmh2; Fc receptor homolog 3; FcRH3; moFcRH3; CD307e; CD307e; Fcrl5; Fcrh3

Immunogen

Expression system for standard: NS0, Immunogen sequence: Q27-A496

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse FCRL5

Subcellular Localization

Cell membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	18	72	133	22	85	154
Standard deviation	1.06	3.17	8.38	1.01	4.93	11.55
CV (%)	5.9%	4.4%	6.3%	4.6%	5.8%	7.5%

Protein Function

It is uncertain whether Met-1 or Met-14 is the initiator.

Background

Fc receptor-like protein 5 is a protein that in humans is encoded by the FCRL5 gene. It is mapped to 3; 3 F1. FCRL5 has also been designated as CD307 (cluster of differentiation 307).

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	18	22	28	29	24	1.62	6.7%
Sample 2	72	78	85	92	82	4.33	5.3%
Sample 3	133	145	1.57	144	145	7.09	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	329693
Gene Name	FCRL5
UniProt ID	Q68SN8
Tissue Specificity	Preferentially expressed in marginal zone B cells.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001482	EK1978	96 wells/kit	$499		Add to Cart

Mouse FCRL5 ELISA Kit PicoKine®

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