Mouse FCRL1 ELISA Kit PicoKine®

Mfr.No.

EK1977

Sensitivity

<50 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.041	0.096	0.166	0.296	0.583	1.027	1.674	2.305

Description

Mouse FCRL1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fcrla in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Fc receptor-like A; Fc receptor homolog expressed in B-cells; Fc receptor-like and mucin-like protein 1; Fc receptor-like protein; Fc receptor-related protein X; FcRX; Fcrla; Fcrl; Fcrl1; Fcrlm1; Fcrx; Freb

Immunogen

Expression system for standard: NS0; Immunogen sequence: A17-S219

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse FCRL1

Subcellular Localization

Cytoplasm.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	90	464	1704	96	476	1814
Standard deviation	4.41	21.81	69.86	5.38	24.28	83.44
CV (%)	4.9%	4.7%	4.1%	5.6%	5.1%	4.6%

Protein Function

May be implicated in B-cell differentiation and lymphomagenesis.

Background

Fc receptor-like protein 1 is a protein that in humans is encoded by the FCRL1 gene. It is mapped to 3; 3 F1.This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alteative splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	90	96	85	88	89	4.02	4.5%
Sample 2	464	409	476	420	442	28.34	6.4%
Sample 3	1704	1617	1595	1596	1628	44.74	2.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	229499
Gene Name	Fcrla
UniProt ID	Q920A9
Tissue Specificity	Highly expressed in spleen. Expressed in immature B-cell and B-cell lines.

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Immunoglobulins,Immunology,Non-CD,Receptors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001483	EK1977	96 wells/kit	$750		Add to Cart

Mouse FCRL1 ELISA Kit PicoKine®

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