Matrices listed below were spiked with certain level of Fasn and the recovery rates were calculated by comparing the measured value to the expected amount of Fasn in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
97
EDTA plasma(n=5)
90-99
95
heparin plasma(n=5)
90-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fasn and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Fasn ELISA Kit allows for the in vitro quantitative determination of Fasn concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Fasn(Fatty acid synthase) ELISA Kit