Mouse ErbB3(V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3) ELISA Kit

Mfr.No.

ELK10786

Sensitivity

0.063 ng/mL

Detection Range

0.16-10 ng/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ErbB3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-105%	86-92%	81-93%	79-97%
EDTA Plasma(n=5)	95-104%	82-93%	81-96%	83-94%
Heparin Plasma(n=5)	80-92%	86-102%	87-103%	85-101%

Standard Curve

Concentration (ng/mL)	OD	Corrected OD
10.00	2.214	2.128
5.00	1.643	1.557
2.50	1.114	1.028
1.25	0.907	0.821
0.63	0.518	0.432
0.32	0.345	0.259
0.16	0.235	0.149
0.00	0.086	0.000

Sample Type

Serum, Plasma and other biological fluids.

Limit Of Detection

10 ng/mL

Precision

Matrices listed below were spiked with certain level of recombinant ErbB3 and the recovery rates were calculated by comparing the measured value to the expected amount of ErbB3 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA Plasma(n=5)	78-96%	87%
Heparin Plasma(n=5)	78-92%	85%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ErbB3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ErbB3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ErbB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ErbB3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

Research Area	Tumor immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15209967	ELK10786	48T	$320		Add to Cart
BHE15209967	ELK10786	96T	$458		Add to Cart

Mouse ErbB3(V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3) ELISA Kit

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