Mouse EPCR ELISA Kit PicoKine®

Mfr.No.

EK1439

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.090	0.186	0.281	0.407	0.712	1.159	1.588	2.092

Description

Mouse EPCR ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Procr in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Endothelial protein C receptor;Activated protein C receptor;APC receptor;Centrocyclin;Centrosomal protein CCD41;Endothelial cell protein C receptor;CD201;Procr;Epcr;

Molecular Weight

27189 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L18-S214

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse EPCR

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Assay Procedure Summary

Activate the sample

Cell culture supeatants, serum and plasma (heparin, EDTA): add activating reagent pro rata, i.e. add 25?l of Solution A into 50?l of sample, 10 min later, add 25?l of Solution B. PH 7.0-7.6.

It is unnecessary to activate the recombinant EPCR.

Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Anticoagulant

heparin or EDTA

Activating Reagent

EPCR is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant EPCR.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	240	1117	4948	231	1074	4505
Standard deviation	14.4	54.73	366.15	16.4	55.84	351.39
CV (%)	6%	4.9%	7.4%	7.1%	5.2%	7.8%

Protein Function

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

Background

Endothelial protein C receptor (EPCR), also known as CCCA or PROCR, is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	240	249	247	239	243	4.32	1.7%
Sample 2	1117	1152	1174	1045	1122	48.88	4.3%
Sample 3	4948	5526	5362	4932	5192	258.64	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	19124
Gene Name	Procr
UniProt ID	Q64695
Protein Name	Endothelial protein C receptor

Applications	ELISA
Research Area	Cardiovascular,Hematopoietic Progenitors,Stem Cells,Vasculature

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001124	EK1439	96 wells/kit	$499		Add to Cart

Mouse EPCR ELISA Kit PicoKine®

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