Mouse EGF PicoKine® Quick ELISA Kit

Mfr.No.

FEK0326

Sensitivity

<1 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Mouse

Sample Volume

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.045	0.190	0.322	0.554	0.989	1.746	2.328	2.403

Description

Mouse EGF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Egf in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N977-R1029

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Egf

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	18	52	277	19	63	274
Standard deviation	1.35	3.43	15.24	0.97	5.1	18.08
CV(%)	7.5%	6.6%	5.5%	5.1%	8.1%	6.6%

Background

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	18	19	19	20	19	1.25	6.6%
Sample 2	52	63	76	72	66	3.81	5.8%
Sample 3	277	256	277	265	269	10.4	3.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	13645
Gene Name	Egf
UniProt ID	P01132

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000338	FEK0326	96 wells/kit	$499		Add to Cart

Mouse EGF PicoKine® Quick ELISA Kit

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