Mouse EFEMP1 ELISA Kit PicoKine®

Mfr.No.

EK1590

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.030	0.086	0.171	0.254	0.383	0.658	1.082	1.886

Description

Mouse EFEMP1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Efemp1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

EGF-containing fibulin-like extracellular matrix protein 1; Fibulin-3; FIBL-3; Efemp1; Fbln3

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q18-F493

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse EFEMP1

Subcellular Localization

Secreted, extracellular space . Secreted, extracellular space, extracellular matrix . Localizes to the lamina propria undeeath the olfactory epithelium.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	685	1294	6512	705	1369	7056
Standard deviation	32.2	71.17	384.21	45.12	91.72	529.2
CV (%)	4.7%	5.5%	5.9%	6.4%	6.7%	7.5%

Protein Function

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth (By similarity).

Background

EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alteatively spliced transcript variants that encode the same protein have been described.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	685	744	804	735	742	40.81	5.5%
Sample 2	1294	1365	1437	1545	1410	88.85	6.3%
Sample 3	6512	7433	6841	8471	7314	416.91	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	216616
Gene Name	Efemp1
UniProt ID	Q8BPB5

Applications	ELISA
Research Area	Calcium Binding Proteins,Calcium Signaling,Neuroscience,Sensory System,Signal Transduction,Signaling Pathway,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001376	EK1590	96 wells/kit	$750		Add to Cart

Mouse EFEMP1 ELISA Kit PicoKine®

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