Mouse DNA Damage-inducible Transcript 3 Protein

Mfr.No.

E1596Mo

Sensitivity

0.09ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of DNA damage-inducible transcript 3 protein (also known as Ddit3) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

AltDDIT3;C/EBP zeta;C/EBP-homologous protein;C/EBP-homologous protein 10;CCAAT/enhancer-binding protein homologous protein;ch;chop;CHOP-;CHOP10;CHOP-10;Ddit3;DDIT-3;DNA damage-inducible transcript 3 protein;gadd15;Growth arrest and DNA-damage-inducible protein GADD153

Molecular Weight

19189

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1596Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	15.45	0.73	4.75
Intra-Assay	2	18	28.43	0.74	2.62
Intra-Assay	3	18	7.08	0.33	4.59

Background

DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotic transcription factor that is encoded by the DDIT3 gene. It is a member of the CCAAT/enhancer-binding protein (C/EBP) family of DNA-binding transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, and has an important role in the cell's stress response.

Protocol

These standard curves of E1596Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	1.864	1.938	1.894
	2.012
16ng/ml	0.957	0.953	0.909
	0.949
8ng/ml	0.436	0.444	0.4
	0.452
4ng/ml	0.301	0.252	0.208
	0.203
2ng/ml	0.169	0.121	0.077
	0.074
0ng/ml	0.055	0.044	0
	0.033

Gene ID	13198
UniProt ID	P35639

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12109438	E1596Mo	48T	$320		Add to Cart
BHE12109438	E1596Mo	96T	$458		Add to Cart

Mouse DNA Damage-inducible Transcript 3 Protein - DDIT3 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse DNA Damage-inducible Transcript 3 Protein - DDIT3 ELISA Kit

Rate it now..!!