Mouse Disintegrin and Metalloproteinase Domain-containing Protein 12

Mfr.No.

E2024Mo

Alternative Names

ADAM 12;ADAM12;Disintegrin and metalloproteinase domain-containing protein 12;Meltrin-alpha

Reactivity

Mouse

Detection Range

0.05-20ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

98504

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.024ng/mL

Background

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.

Gene ID	11489
UniProt ID	Q61824
Antigen	ADAM12

Research Area

Signal Transduction; Cancer

Protocol

These standard curves of E2024Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.282	2.199	2.163
	2.116
6ng/mL	1.331	1.394	1.358
	1.456
3ng/mL	0.679	0.641	0.605
	0.603
1.5ng/mL	0.367	0.397	0.361
	0.428
0.75ng/mL	0.124	0.151	0.115
	0.179
0ng/mL	0.04	0.036	0
	0.032

Precision

We measured random samples of E2024Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.7	0.09	5.2
Intra-Assay	2	18	3.6	0.17	4.8
Intra-Assay	3	18	5.4	0.14	2.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109835	E2024Mo	48T	$320		Add to Cart
BHE12109835	E2024Mo	96T	$458		Add to Cart

Mouse Disintegrin and Metalloproteinase Domain-containing Protein 12 - ADAM12 ELISA Kit

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