Mouse Dihydrofolate Reductase

Mfr.No.

E2008Mo

Alternative Names

DHFR;Dihydrofolate reductase

Reactivity

Mouse

Detection Range

20-3500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

21606

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.42ng/L

Background

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway (PubMed: 25980602). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (PubMed: 25980602). Binds its own mRNA.

Gene ID	13361
UniProt ID	P00375
Antigen	DHFR

Research Area

Signal Transduction

Protocol

These standard curves of E2008Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.106	2.125	2.079
	2.144
1000ng/L	1.516	1.457	1.411
	1.398
500ng/L	0.559	0.656	0.61
	0.754
250ng/L	0.433	0.386	0.34
	0.338
125ng/L	0.126	0.132	0.086
	0.138
0ng/L	0.053	0.046	0
	0.039

Precision

We measured random samples of E2008Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	593.9	23.52	4
Intra-Assay	2	18	1324.6	43.95	3.3
Intra-Assay	3	18	278.5	11.06	4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109820	E2008Mo	48T	$320		Add to Cart
BHE12109820	E2008Mo	96T	$458		Add to Cart

Mouse Dihydrofolate Reductase - DHFR ELISA Kit

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