Mouse Dickkopf-related Protein 1

Mfr.No.

E2006Mo

Sensitivity

14.12ng/L

Detection Range

30-9000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Dickkopf-1;Dickkopf-related protein 1;DKK 1;DKK1;Dkk-1;mDkk-1

Molecular Weight

29298

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2006Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	548.2	29.26	5.3
Intra-Assay	2	18	1848.2	98.49	5.3
Intra-Assay	3	18	1033.6	50.25	4.9

Background

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed: 18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed: 26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed: 18505822).

Protocol

These standard curves of E2006Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.175	2.231	2.183
	2.287
2400ng/L	1.866	1.884	1.836
	1.902
1200ng/L	1.165	1.138	1.089
	1.111
600ng/L	0.642	0.639	0.591
	0.635
300ng/L	0.428	0.407	0.359
	0.386
0ng/L	0.032	0.048	0
	0.065

Gene ID	13380
UniProt ID	O54908

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109818	E2006Mo	48T	$320		Add to Cart
BHE12109818	E2006Mo	96T	$458		Add to Cart

Mouse Dickkopf-related Protein 1 - DKK1 ELISA Kit

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