Mouse Dickkopf-like Protein 1

Mfr.No.

E1215Mo

Alternative Names

Dickkopf-like protein 1;DKKL 1;DKKL1;Protein soggy-1;SGY-1

Reactivity

Mouse

Detection Range

20-7000ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

26639

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.47ng/L

Background

Involved in fertilization by facilitating sperm penetration of the zona pellucida (PubMed: 19596312, PubMed: 22817830). May promote spermatocyte apoptosis, thereby limiting sperm production. In adults, may reduce testosterone synthesis in Leydig cells (PubMed: 18818293). Is not essential either for development or fertility (PubMed: 19596310).

Gene ID	50722
UniProt ID	Q9QZL9
Antigen	DKKL1

Research Area

Others

Protocol

These standard curves of E1215Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/L	2.044	2.159	2.102
	2.274
2000ng/L	1.513	1.62	1.563
	1.726
1000ng/L	0.99	1.038	0.981
	1.087
500ng/L	0.629	0.658	0.601
	0.687
250ng/L	0.453	0.447	0.39
	0.442
0ng/L	0.054	0.057	0
	0.061

Precision

We measured random samples of E1215Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1606.4	76.59	4.8
Intra-Assay	2	18	363.5	24.51	6.7
Intra-Assay	3	18	876.6	57.68	6.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109080	E1215Mo	48T	$320		Add to Cart
BHE12109080	E1215Mo	96T	$458		Add to Cart

Mouse Dickkopf-like Protein 1 - DKKL1 ELISA Kit

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