Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine®

Mfr.No.

EK2008

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.067	0.111	0.147	0.231	0.391	0.700	1.345	2.026

Description

Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf23 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 23; Decoy TRAIL receptor 1; TNF receptor family member SOB; TNF receptor homolog 1; Tumor necrosis factor receptor p60 homolog 1; Tnfrsf23; Dctrailr1; Tnfrh1; Tnfrsf1al1

Immunogen

Expression system for standard: NS0; Immunogen sequence: M30-C155

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF23

Subcellular Localization

Cell membrane. GPI-anchor.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	31	118	452	33	124	468
Standard deviation	1.49	5.55	22.15	1.68	6.70	31.36
CV (%)	4.8%	4.7%	4.9%	5.1%	5.4%	6.7%

Protein Function

Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis through ligand competition. Cannot induce the NF-kappa-B pathway.

Background

Mouse TNFRSF23, also known as TNFRH1 and DcTRAIL R1, is a GPI-anchored mouse TNF receptor superfamily member that lacks a cytoplasmic death domain and does not induce apoptosis. This gene is mapped to 7 F5; 7 88.28 Cm. This gene encodes a member of the tumor necrosis factor superfamily of proteins. The encoded receptor has been shown to bind to the ligand TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), but to have no signaling capacity. This gene shows elevated expression in mice with diet-induced fatty liver disease. This gene and other family members are present in a gene cluster on chromosome 7. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	31	32	37	34	33	2.29	6.9%
Sample 2	118	121	115	107	115	5.21	4.5%
Sample 3	452	460	462	460	458	3.84	0.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	79201
Antigen	DcTRAILR1/TNFRSF23
Gene Name	Tnfrsf23
UniProt ID	Q9ER63
Protein Name	Tumor necrosis factor receptor superfamily member 23
Tissue Specificity	Ubiquitous.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001543	EK2008	96 wells/kit	$750		Add to Cart

Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine®

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