Mouse DAN/NBL1 ELISA Kit PicoKine®

Mfr.No.

EK0747

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.049	0.075	0.104	0.204	0.464	0.838	1.386	2.028

Description

Mouse DAN/NBL1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Nbl1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neuroblastoma suppressor of tumorigenicity 1;N03;Zinc finger protein DAN;Nbl1;Dan, Dana;

Molecular Weight

19107 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A17-D178

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse DAN

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	84	591	1510	86	596	1475
Standard deviation	5.54	31.91	78.52	6.7	37.54	89.97
CV (%)	6.6%	5.4%	5.2%	7.8%	6.3%	6.1%

Protein Function

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Background

Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	84	77	79	90	82	5.02	6.1%
Sample 2	591	589	657	668	626	36.46	5.8%
Sample 3	1510	1598	1670	1616	1598	57.55	3.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17965
Gene Name	Nbl1
UniProt ID	Q61477
Protein Name	Neuroblastoma suppressor of tumorigenicity 1

Applications	ELISA
Research Area	Cancer,Growth Factors/Hormones,Oncoproteins/Suppressors,Signal Transduction,Tumor Suppressors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000979	EK0747	96 wells/kit	$499		Add to Cart

Mouse DAN/NBL1 ELISA Kit PicoKine®

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