Mouse CXCL9/Mig ELISA Kit PicoKine®

Mfr.No.

EK0733

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.012	0.031	0.074	0.157	0.305	0.587	1.032	1.901

Description

Mouse CXCL9/Mig ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cxcl9 in cell culture supeatants, serum and plasma (EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-X-C motif chemokine 9;Gamma-interferon-induced monokine;Monokine induced by interferon-gamma;MIG;MuMIG;Protein m119;Small-inducible cytokine B9;Cxcl9;Mig, Scyb9;

Molecular Weight

14444 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T22-T126

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CXCL9

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

EDTA

Activating Reagent

Aliquot 0.1 ml per well of the 4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml mouse CXCL9 standard solutions into the precoated 96-well plate. Add 0.1 ml of the sample diluent buffer into the control well (Zero well). Add 0.1 ml of each properly diluted sample of mouse cell culture supeatants, serum or plasma(EDTA) to each empty well. See Sample Dilution Guideline above for details. It is recommended that each mouse CXCL9 standard solution and each sample be measured in duplicate.

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	95	297	1865	96	268	1558
Standard deviation	3.89	9.92	98.66	4.86	9.11	36.77
CV(%)	4.1%	3.3%	5.3%	5.1%	3.4%	2.4%

Protein Function

May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response.

Background

Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-?. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	86	84	92	82	86	3.74	4.3%
Sample 2	228	207	217	212	216	7.77	3.5%
Sample 3	891	891	830	879	872	25.16	2.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17329
Gene Name	Cxcl9
UniProt ID	P18340
Protein Name	C-X-C motif chemokine 9

Applications	ELISA
Research Area	Chemokines,Immunology,Innate Immunity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001009	EK0733	96 wells/kit	$499		Add to Cart

Mouse CXCL9/Mig ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse CXCL9/Mig ELISA Kit PicoKine®

Rate it now..!!