Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine®

Mfr.No.

EK0919

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.001	0.065	0.138	0.303	0.530	1.091	1.601	1.900

Description

Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cxcl5 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-X-C motif chemokine 5;Cytokine LIX;Small-inducible cytokine B5;GCP-2 (1-78);GCP-2 (9-78);Cxcl5;Scyb5;

Molecular Weight

14235 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V45-A118

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CXCL5

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	35	166	388	36	150	360
Standard deviation	2.17	8.13	16.93	2.48	8.1	20.52
CV (%)	6.2%	4.9%	4.7%	6.9%	5.4%	5.7%

Protein Function

May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2 (1-78) and, more potent, GCP- 2 (9-78) attract neutrophils and are involved in neutrophil activation. .

Background

C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunbu pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	35	34	32	34	33	1.08	3.2%
Sample 2	166	180	162	152	165	10.04	6%
Sample 3	388	401	390	458	409	28.57	6.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20311
Gene Name	CXCL5
UniProt ID	P50228
Protein Name	C-X-C motif chemokine 5

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Chemokines,Immunology,Innate Immunity,Macrophage/Inflammation,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000695	EK0919	96 wells/kit	$499		Add to Cart

Mouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine®

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