Mouse CXCL10/IP-10 ELISA Kit PicoKine®

Mfr.No.

EK0736

Sensitivity

<1 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.044	0.072	0.104	0.162	0.292	0.580	1.126	2.069

Description

Mouse CXCL10/IP-10 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cxcl10 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein;Gamma-IP10;IP-10;C7;Interferon-gamma induced protein CRG-2;Small-inducible cytokine B10;Cxcl10;Crg2, Ifi10, Inp10, Scyb10;

Molecular Weight

10789 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I22-P98

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CXCL10

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	105	245	923	110	248	879
Standard deviation	10.237	13.72	47.99	10.23	18.6	56.25
CV (%)	7.5%	5.6%	5.2%	9.3%	7.5%	6.4%

Protein Function

In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development.

Background

Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	105	98	104	102	102	2.68	2.6%
Sample 2	245	274	275	259	263	12.29	4.6%
Sample 3	923	908	845	862	884	32.01	3.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	15945
Antigen	CXCL10/IP-10
Gene Name	CXCL10
UniProt ID	P17515
Protein Name	C-X-C motif chemokine 10
Tissue Specificity	Expressed in the spleen, thymus, lymph nodes and liver. .

Applications	ELISA
Research Area	Angiogenesis,Cancer,Chemokines,Immunology,Innate Immunity,Invasion/Microenvironment

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000594	EK0736	96 wells/kit	$499		Add to Cart

Mouse CXCL10/IP-10 ELISA Kit PicoKine®

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