Matrices listed below were spiked with certain level of Ctsl and the recovery rates were calculated by comparing the measured value to the expected amount of Ctsl in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
93
EDTA plasma(n=5)
85-103
96
heparin plasma(n=5)
85-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ctsl and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Ctsl ELISA Kit allows for the in vitro quantitative determination of Ctsl concentrations in serum, plasma, tissue homogenates and other biological fluids.