Mouse Cripto/TDGF1 ELISA Kit PicoKine®

Mfr.No.

EK1405

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.079	0.138	0.181	0.283	0.446	0.764	1.354	2.050

Description

Mouse Cripto/TDGF1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tdgf1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Teratocarcinoma-derived growth factor;Cripto growth factor;Epidermal growth factor-like Cripto protein;Tdgf1;Cripto;

Molecular Weight

18754 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: R26-Q150

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Cripto

Subcellular Localization

Cell membrane ; Lipid-anchor, GPI-anchor .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	110	661	1213	107	721	1176
Standard deviation	7.5%7	27.1	73.99	4.7%4	37.49	84.9%
CV (%)	4.7%	4.1%	6.1%	4.9%	5.2%	7.5%

Protein Function

Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

Background

Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alteate splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	110	110	114	115	112	2.27	2%
Sample 2	661	633	642	685	655	19.92	3%
Sample 3	1213	1047	1166	1231	1164	71.73	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21667
Gene Name	Tdgf1
UniProt ID	P51865
Protein Name	Teratocarcinoma-derived growth factor
Tissue Specificity	Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus.

Applications	ELISA
Research Area	Embryonic Stem Cells,Lineage Markers,Lineage Specification,Secreted,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001001	EK1405	96 wells/kit	$499		Add to Cart

Mouse Cripto/TDGF1 ELISA Kit PicoKine®

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