Mouse Contactin-4 ELISA Kit PicoKine®

Mfr.No.

EK1781

Sensitivity

<50 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.057	0.101	0.156	0.256	0.456	0.896	1.592	2.256

Description

Mouse Contactin-4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cntn4 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Contactin-4; Brain-derived immunoglobulin superfamily protein 2; BIG-2; Cntn4; Kiaa3024

Immunogen

Expression system for standard: NS0; Immunogen sequence: D19-S1000

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Contactin-4

Subcellular Localization

Secreted. Cell membrane. GPI-anchor.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	451	1849	7615	513	2075	8739
Standard deviation	26.61	79.51	312.22	33.86	118.28	681.64
CV (%)	5.9%	4.3%	4.1%	6.6%	5.7%	7.8%

Protein Function

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.

Background

Contactin-4 is a protein that in humans is encoded by the CNTN4 gene. It is mapped to 6; 6 E1. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Several alteatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	451	511	512	479	488	25.27	5.1%
Sample 2	1849	2120	1840	2095	1976	131.83	6.6%
Sample 3	7615	7780	8028	8212	7908	228.60	2.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	269784
Gene Name	Cntn4
UniProt ID	Q69Z26
Tissue Specificity	Expressed in the region of developing olfactory neurons. Isoform 2 is expressed in mature sensory cells of the vomeronasal neuroepithelium and at lower level in olfactory neuroepithelium.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001605	EK1781	96 wells/kit	$750		Add to Cart

Mouse Contactin-4 ELISA Kit PicoKine®

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