Mouse Coagulation Factor 7

Mfr.No.

E1470Mo

Description

This sandwich kit is for the accurate quantitative detection of Coagulation Factor VII (also known as F7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Coagulation factor VII;F7;Factor VII heavy chain;Factor VII light chain;Serum prothrombin conversion accelerator

Reactivity

Mouse

Detection Range

0.2-60ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

50276

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.116ng/mL

Background

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).

Gene ID	14068
UniProt ID	P70375
Antigen	F7

Research Area

Blood Coagulation

Protocol

These standard curves of E1470Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/mL	2.298	2.221	2.161
	2.144
16ng/mL	1.206	1.206	1.146
	1.205
8ng/mL	0.654	0.726	0.666
	0.797
4ng/mL	0.534	0.509	0.449
	0.484
2ng/mL	0.179	0.202	0.142
	0.225
0ng/mL	0.059	0.06	0
	0.061

Precision

We measured random samples of E1470Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	13.8	0.68	4.9
Intra-Assay	2	18	20.5	0.72	3.5
Intra-Assay	3	18	6.9	0.45	6.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109327	E1470Mo	48T	$320		Add to Cart
BHE12109327	E1470Mo	96T	$458		Add to Cart

Mouse Coagulation Factor 7 - F7 ELISA Kit

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