Mouse CIC(Circulating Immune Complex) ELISA Kit

Mfr.No.

ELK0099

Sensitivity

4.81 ng/mL

Detection Range

15.63-1000 ng/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CIC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	86-96%	93-104%	89-97%	85-94%
EDTA Plasma(n=5)	89-99%	83-95%	98-104%	96-106%
Heparin Plasma(n=5)	85-100%	84-98%	95-101%	88-102%

Standard Curve

Concentration (ng/mL)	OD	Corrected OD
1000.00	2.035	1.937
500.00	1.527	1.429
250.00	0.974	0.876
125.00	0.781	0.683
62.50	0.563	0.465
31.25	0.379	0.281
15.63	0.225	0.127
0.00	0.098	0.000

Sample Type

Serum, Plasma

Limit Of Detection

1000 ng/mL

Precision

Matrices listed below were spiked with certain level of recombinant CIC and the recovery rates were calculated by comparing the measured value to the expected amount of CIC in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA Plasma(n=5)	94-107%	100%
Heparin Plasma(n=5)	87-95%	91%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CIC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CIC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CIC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CIC in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

Research Area	Metabolic pathway;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208984	ELK0099	48T	$320		Add to Cart
BHE15208984	ELK0099	96T	$458		Add to Cart

Mouse CIC(Circulating Immune Complex) ELISA Kit

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