Mouse CD99L2 ELISA Kit PicoKine®

Mfr.No.

EK2091

Sensitivity

<50 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.062	0.144	0.218	0.378	0.612	0.939	1.446	2.059

Description

Mouse CD99L2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd99l2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CD99 antigen-like protein 2; MIC2-like protein 1; CD99; Cd99l2; Mic2l1

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D26-G161

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD99L2

Subcellular Localization

Cell membrane. Single-pass type I membrane protein. Extracellular side. Cell junction.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	467	1310	7832	529	2102	8406
Standard deviation	19.15	56.33	360.27	30.68	145.04	605.23
CV (%)	4.1%	4.3%	4.6%	5.8%	6.9%	7.2%

Protein Function

Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.

Background

CD99 antigen-like protein 2 is a protein that in humans is encoded by the CD99L2 gene. It is mapped to X; X A7.3. This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alteate splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	467	427	448	472	453	17.72	3.9%
Sample 2	1310	1396	1545	1357	1402	87.99	6.2%
Sample 3	7832	7176	7216	8274	7624	456.32	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	171486
Gene Name	Cd99l2
UniProt ID	Q8BIF0
Tissue Specificity	Highly expressed in the nervous system, including brain, dentate nucleus of hippocampus, granular and Purkinje cells of cerebellum, brain stem nucleus and choroid plexus. Expressed in peripheral blood T- and B-cells and neutrophils. Almost undetectable in bone marrow-derived neutrophils. Also expressed in thymocytes with higher expression in cortical thymocytes than in medullary thymocytes. Expressed at high levels in testis (mostly in germ cells and Sertoli cells) and ovary (mostly in granulosa cells). Expressed in lung, heart, kidney and liver; however, expression in heart, kidney and liver seems restricted to endothelial cells. Highly expressed in endothelial cells and to a lower level in vascular smooth muscle cells. Low expression in spleen.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001637	EK2091	96 wells/kit	$750		Add to Cart

Mouse CD99L2 ELISA Kit PicoKine®

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