Mouse CD99 ELISA Kit PicoKine®

Mfr.No.

EK2089

Sensitivity

<50 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.110	0.219	0.378	0.541	0.885	1.190	1.787	2.465

Description

Mouse CD99 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd99 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CD99 antigen; Paired immunoglobin-like type 2 receptor-ligand; PILR-L; CD99; Cd99; Pilrl

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D29-G137

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD99

Subcellular Localization

Membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	153	549	2842	214	654	3861
Standard deviation	6.27	23.61	130.73	10.91	38.59	258.69
CV (%)	4.1%	4.3%	4.6%	5.1%	5.9%	6.7%

Protein Function

Involved in T-cell adhesion processes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1.

Background

CD99 antigen (Cluster of differentiation 99), also known as MIC2 or single-chain type-1 glycoprotein, is a heavily O-glycosylated transmembrane protein that is encoded by the CD99 gene in humans. It is mapped to 4; 4. The protein has a mass of 32 kD. Unusually for a gene present on the X chromosome, the CD99 gene does not undergo X inactivation, and it was the first such pseudoautosomal gene to be discovered in humans.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	153	167	180	162	165	9.75	5.9%
Sample 2	549	593	548	547	559	19.49	3.4%
Sample 3	2842	2852	2882	2685	2815	76.62	2.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	673094
Gene Name	Cd99
UniProt ID	Q8VCN6
Tissue Specificity	Widely expressed with high levels in lung, spleen, thymus, liver and spinal chord. Expressed on leukocytes and endothelial cell contacts. Detected in a wide range of T-cells, including CD4-/CD8- and CD4+/CD8+ thymocytes. Expression is much lower in peripheral T-cells than in thymocytes.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001635	EK2089	96 wells/kit	$499		Add to Cart

Mouse CD99 ELISA Kit PicoKine®

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