Mouse CD86/B7-2 ELISA Kit PicoKine®

Mfr.No.

EK0711

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.067	0.125	0.17	0.307	0.525	0.897	1.449	2.211

Description

Mouse CD86/B7-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd86 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

T-lymphocyte activation antigen CD86;Activation B7-2 antigen;Early T-cell costimulatory molecule 1;ETC-1;CD86;Cd86;

Molecular Weight

34666 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: V26-G245

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD86

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	179	772	1639	165	834	1677
Standard deviation	11.09	412.546	86.86	12.54	62.55	95.58
CV (%)	6.2%	6.2%	5.3%	7.6%	7.5%	5.7%

Protein Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation.

Background

Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	179	175	168	168	172	4.71	2.7%
Sample 2	772	787	740	767	766	16.97	2.2%
Sample 3	1639	1511	1669	1837	1664	116.17	6.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12524
Gene Name	Cd86
UniProt ID	P42082
Protein Name	T-lymphocyte activation antigen CD86
Tissue Specificity	Expressed on activated B-cells.

Applications	ELISA
Research Area	Adaptive Immunity,Cell Type Markers,Hematopoietic Progenitors,Host-Virus Interaction,Immunology,Interspecies Interaction,Lymphoid,Microbiology,Myeloid,Stem Cells,T Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000975	EK0711	96 wells/kit	$499		Add to Cart

Mouse CD86/B7-2 ELISA Kit PicoKine®

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