Mouse CD73/NT5E ELISA Kit PicoKine®

Mfr.No.

EK2131

Sensitivity

<20 pg/ml

Detection Range

0.156 ng/ml - 10 ng/ml

Reactivity

Mouse

Sample Volume

Concentration(ng/ml)	0	0.156	0.312	0.625	1.25	2.5	5	10
O.D.	0.019	0.138	0.241	0.334	0.409	0.719	1.304	1.902

Description

Mouse CD73/NT5E ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Nt5e in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Nt5e; Nt5; Nte; 5'-nucleotidase; 5'-NT; EC 3.1.3.5; Ecto-5'-nucleotidase; CD antigen CD73

Immunogen

Expression system for standard: CHO; Immunogen sequence: W29-S551

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Nt5e

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	203	987	3240	274	1150	3163
Standard deviation	8.93	40.47	165.24	15.62	73.60	211.92
CV(%)	4.4%	4.1%	5.1%	5.7%	6.4%	6.7%

Background

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the exteal face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	203	291	294	288	269	5.92	2.2%
Sample 2	987	1055	1152	756	988	19.75	2.0%
Sample 3	3240	3451	4010	4526	3807	95.17	2.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	23959
Gene Name	Nt5e
UniProt ID	Q61503
Protein Name	5'-nucleotidase

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001677	EK2131	96 wells/kit	$499		Add to Cart

Mouse CD73/NT5E ELISA Kit PicoKine®

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