Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine®

Mfr.No.

EK1310

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.055	0.137	0.210	0.357	0.676	1.174	1.633	2.033

Description

Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd70 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CD70 antigen;CD27 ligand;CD27-L;Tumor necrosis factor ligand superfamily member 7;CD70;Cd70;Cd27l, Cd27lg, Tnfsf7;

Molecular Weight

21919 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S45-P195

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD70

Subcellular Localization

Membrane; Single-pass type II membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	79	248	791	80	240	839
Standard deviation	3.71	18.35	60.9	3.92	19.92	75.51
CV (%)	4.7%	7.4%	7.7%	4.9%	8.3%	9%

Protein Function

Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. .

Background

CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	79	82	85	75	80	3.69	4.6%
Sample 2	248	239	232	248	241	6.72	2.7%
Sample 3	791	821	875	932	854	53.8	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21948
Gene Name	CD70
UniProt ID	O55237
Protein Name	CD70 antigen
Tissue Specificity	Very low level of expression. Detected in splenocytes and thymocytes.

Applications	ELISA
Research Area	Cancer,Cytokines,Growth Factors,Hematopoietic Progenitors,Immunology,Innate Immunity,Lymphoid,Stem Cells,T Lymphocytic Lineage,TNF Superfamily

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000901	EK1310	96 wells/kit	$499		Add to Cart

Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine®

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