Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine®

Mfr.No.

EK1223

Sensitivity

<20 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.001	0.148	0.241	0.496	0.863	1.214	1.580	1.834

Description

Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ncam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neural cell adhesion molecule 1;N-CAM-1;NCAM-1;CD56;Ncam1;Ncam;

Molecular Weight

119427 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-T711

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD56

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1344	4632	15378	1401	4645	16195
Standard deviation	90.04	342.76	953.43	120.48	422.69	1214.62
CV (%)	6.7%	7.4%	6.2%	8.6%	9.1%	7.5%

Protein Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Background

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellcell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1344	1609	1386	1481	1455	101.82	6.9%
Sample 2	4632	4852	4530	4967	4745	173	3.6%
Sample 3	15378	17338	16502	16161	16344	703.49	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17967
Antigen	NCAM-1
Gene Name	NCAM1
UniProt ID	P13595
Protein Name	Neural cell adhesion molecule 1
Sequence	Contains 2 fibronectin type-III domains.

Applications	ELISA
Research Area	Axonal Guidance Proteins,Cell Adhesion Proteins,Cell Type Marker,Developmental Biology,ECM Proteins,Growth and Development,Hematopoietic Progenitors,Membrane Proteins,Nervous System Development,Neural Signal Transduction,Neurology Process,Neuron Marker,Neuroscience,Organogenesis,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000835	EK1223	96 wells/kit	$499		Add to Cart

Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine®

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