Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine®

Mfr.No.

EK1223

Sensitivity

<20 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.001	0.148	0.241	0.496	0.863	1.214	1.580	1.834

Description

Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ncam1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neural cell adhesion molecule 1;N-CAM-1;NCAM-1;CD56;Ncam1;Ncam;

Molecular Weight

119427 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-T711

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD56

Subcellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1344	4632	15378	1401	4645	16195
Standard deviation	90.04	342.76	953.43	120.48	422.69	1214.62
CV (%)	6.7%	7.4%	6.2%	8.6%	9.1%	7.5%

Protein Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Background

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alteatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellcell adhesion, neurite outgrowth, synaptic plasticity, and leaing and memory.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1344	1609	1386	1481	1455	101.82	6.9%
Sample 2	4632	4852	4530	4967	4745	173	3.6%
Sample 3	15378	17338	16502	16161	16344	703.49	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17967
Gene Name	NCAM1
UniProt ID	P13595
Protein Name	Neural cell adhesion molecule 1
Sequence	Contains 2 fibronectin type-III domains.

Applications	ELISA
Research Area	Axonal Guidance Proteins,Cell Adhesion Proteins,Cell Type Marker,Developmental Biology,ECM Proteins,Growth and Development,Hematopoietic Progenitors,Membrane Proteins,Nervous System Development,Neural Signal Transduction,Neurology Process,Neuron Marker,Neuroscience,Organogenesis,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000835	EK1223	96 wells/kit	$499		Add to Cart

Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine®

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