Mouse CD55 ELISA Kit PicoKine®

Mfr.No.

EK1949

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.134	0.176	0.203	0.259	0.377	0.667	1.200	1.989

Description

Mouse CD55 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd55 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Complement decay-accelerating factor; GPI-anchored; DAF-GPI; CD55; Cd55

Immunogen

Expression system for standard: NS0; Immunogen sequence: D35-G362

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD55

Subcellular Localization

Cell membrane, GPI-anchor

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	225	925	4690	308	1190	4853
Standard deviation	9.45	39.78	192.29	20.33	80.92	344.56
CV (%)	4.2%	4.3%	4.1%	6.6%	6.8%	7.1%

Protein Function

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.

Background

Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. It is mapped to 1 E4; 1 56.89 cM. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alteative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alteatively spliced transcripts may produce soluble proteins.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	225	203	199	214	210	10.13	4.8%
Sample 2	925	1079	935	978	979	60.93	6.2%
Sample 3	4690	4298	4040	4458	4371	236.75	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	13136
Gene Name	Cd55
UniProt ID	Q61475
Tissue Specificity	Brain, secretory epithelia, skeletal muscle, liver, testes, thymus, spleen and lymph node.

Applications	ELISA
Research Area	Cell Type Markers,Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001466	EK1949	96 wells/kit	$499		Add to Cart

Mouse CD55 ELISA Kit PicoKine®

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