Mouse CD30L ELISA Kit PicoKine®

Mfr.No.

EK0572

Sensitivity

<6 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.112	0.112	0.133	0.203	0.326	0.594	1.146	2.091

Description

Mouse CD30L ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfsf8 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor ligand superfamily member 8;CD30 ligand;CD30-L;CD153;Tnfsf8;Cd30l, Cd30lg;

Molecular Weight

26519 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q68-D239

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD30L

Subcellular Localization

Membrane; Single-pass type II membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	35	254	445	38	267	477
Standard deviation	2.24	16.51	34.26	2.58	21.09	43.4
CV (%)	6.4%	6.5%	7.7%	6.8%	7.9%	9.1%

Protein Function

Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

Background

The human CD30L (CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154 (CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this"reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	35	30	34	31	32	2.06	6.4%
Sample 2	254	260	260	224	249	14.92	5.9%
Sample 3	445	507	470	490	478	23.11	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21949
Gene Name	TNFSF8
UniProt ID	P32972
Protein Name	Tumor necrosis factor ligand superfamily member 8

Applications	ELISA
Research Area	Adaptive Immunity,Immunology,T Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000525	EK0572	96 wells/kit	$499		Add to Cart

Mouse CD30L ELISA Kit PicoKine®

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