Mouse CD300b ELISA Kit PicoKine®

Mfr.No.

EK1865

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.070	0.131	0.197	0.312	0.564	0.793	1.212	1.825

Description

Mouse CD300b ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd300lb in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CMRF35-like molecule 7; CLM-7; CD300 antigen-like family member B; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; CD300b; Cd300lb; Irem3; Lmir5

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I18-P209

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD300b

Subcellular Localization

Cell membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	19	70	278	23	118	418
Standard deviation	0.78	3.29	13.62	1.17	6.49	26.33
CV (%)	4.1%	4.7%	4.9%	5.1%	5.5%	6.3%

Protein Function

Acts as an activating immune receptor in mast cells through its interaction with ITAM-bearing adapter TYROBP.

Background

CD300 molecule like family member b is a protein that in humans is encoded by the CD300LB gene. It is mapped to 11; 11 E2. CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	19	21	21	19	20	1.00	5.0%
Sample 2	70	67	57	66	65	4.84	7.4%
Sample 3	278	270	278	255	270	9.39	3.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	217304
Gene Name	Cd300lb
UniProt ID	Q3U497
Tissue Specificity	Expressed in myeloid cells.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001623	EK1865	96 wells/kit	$750		Add to Cart

Mouse CD300b ELISA Kit PicoKine®

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