Mouse CD30/TNFRSF8 ELISA Kit PicoKine®

Mfr.No.

EK0570

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.014	0.081	0.148	0.235	0.505	1.010	1.482	2.158

Description

Mouse CD30/TNFRSF8 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf8 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 8;CD30L receptor;Lymphocyte activation antigen CD30;CD30;Tnfrsf8;

Molecular Weight

53216 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: F19-T281

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD30

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supernatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	40	255	414	42	257	418
Standard deviation	2.6	16.83	13.19	3.19	20.56	25.49
CV (%)	6.5%	6.6%	4.3%	7.6%	8%	6.1%

Protein Function

Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B (By similarity). .

Background

CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	40	35	38	39	38	1.87	4.9%
Sample 2	255	232	265	245	249	12.21	4.9%
Sample 3	414	393	421	433	415	14.53	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21941
Antigen	CD30/TNFRSF8
Gene Name	TNFRSF8
UniProt ID	Q60846
Protein Name	Tumor necrosis factor receptor superfamily member 8
Tissue Specificity	Detected in thymus and in activated splenocytes.

Applications	ELISA
Research Area	Cancer,Cell Type Markers,Cytokines,Immunology,Innate Immunity,Receptors,TNF Superfamily,Tumor Biomarkers,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000524	EK0570	96 wells/kit	$499		Add to Cart

Mouse CD30/TNFRSF8 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse CD30/TNFRSF8 ELISA Kit PicoKine®

Rate it now..!!