Mouse CD23/FCER2 ELISA Kit PicoKine®

Mfr.No.

EK0924

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.021	0.045	0.137	0.279	0.509	1.009	1.503	2.041

Description

Mouse CD23/FCER2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fcer2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Low affinity immunoglobulin epsilon Fc receptor;Fc-epsilon-RII;Lymphocyte IgE receptor;CD23;Fcer2;Fcer2a;

Molecular Weight

37648 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E50-P331

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD23

Subcellular Localization

Cell membrane ; Single-pass type II membrane protein . Cell membrane ; Lipid-anchor .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	256	1831	5853	234	1881	5493
Standard deviation	11	137.32	345.32	12.87	156.12	373.52
CV (%)	4.3%	7.5%	5.9%	5.5%	8.3%	6.8%

Protein Function

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

Background

CD23, also known as Fc epsilon RII, or Fc?RII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	256	296	293	254	274	19.79	7.2%
Sample 2	1831	1672	1609	1822	1733	95.68	5.5%
Sample 3	5853	5387	5221	5268	5432	250.34	4.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	14128
Gene Name	Fcer2
UniProt ID	P20693
Protein Name	Low affinity immunoglobulin epsilon Fc receptor

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunoglobulins,Immunology,Lymphoid,Receptors,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001039	EK0924	96 wells/kit	$499		Add to Cart

Mouse CD23/FCER2 ELISA Kit PicoKine®

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