Mouse CCL9/MIP-1 gamma ELISA Kit PicoKine®

Mfr.No.

EK1225

Sensitivity

<1 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.030	0.111	0.197	0.369	0.680	1.252	1.783	2.150

Description

Mouse CCL9/MIP-1 gamma ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl9 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 9;CCF18;Macrophage inflammatory protein 1-gamma;MIP-1-gamma;Macrophage inflammatory protein-related protein 2;MRP-2;Small-inducible cytokine A9;CCL9 (29-101);CCL9 (30-101);CCL9 (31-101);Ccl9;Mrp2, Scya10, Scya9;

Molecular Weight

13871 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q22-Q122

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CCL9

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	12	77	272	12	74	290
Standard deviation	0.86	4.69	19.04	0.9	4.88	22.33
CV (%)	7.2%	6.1%	7%	7.5%	6.6%	7.7%

Protein Function

Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.

Background

Chemokine (C-C motif) ligand 9 (CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium (FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1 (the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	12	12	12	11	11	0.43	3.9%
Sample 2	77	67	78	82	76	5.52	7.2%
Sample 3	272	286	279	302	284	11.12	3.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20308
Gene Name	Ccl9
UniProt ID	P51670
Protein Name	C-C motif chemokine 9
Tissue Specificity	Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Chemokines,Immunology,Innate Immunity,Macrophage/Inflammation,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000837	EK1225	96 wells/kit	$499		Add to Cart

Mouse CCL9/MIP-1 gamma ELISA Kit PicoKine®

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