Mouse Cathepsin K - CTSK ELISA Kit

Mfr.No.

E0852Mo

Description

This sandwich kit is for the accurate quantitative detection of Cathepsin K (also known as CTSK) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cathepsin K;CTSK

Reactivity

Mouse

Detection Range

0.3-90ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

36889

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.153ng/mL

Background

Thiol protease involved in osteoclastic bone resorption. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation (By similarity). Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (PubMed: 12782676).

Gene ID	13038
UniProt ID	P55097
Antigen	CTSK

Research Area

Signal Transduction

Protocol

These standard curves of E0852Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.09	2.1	2.069
	2.109
24ng/mL	1.31	1.3	1.27
	1.29
12ng/mL	0.702	0.628	0.598
	0.555
6ng/mL	0.388	0.365	0.335
	0.343
3ng/mL	0.204	0.188	0.157
	0.172
0ng/mL	0.033	0.03	0
	0.027

Precision

We measured random samples of E0852Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.6	0.5	5.8
Intra-Assay	2	18	13.1	0.63	4.8
Intra-Assay	3	18	32.6	1.02	3.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108724	E0852Mo	48T	$320		Add to Cart
BHE12108724	E0852Mo	96T	$458		Add to Cart

Mouse Cathepsin K - CTSK ELISA Kit

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