Mouse Cathepsin E ELISA Kit PicoKine®

Mfr.No.

EK2128

Sensitivity

<20 pg/ml

Detection Range

0.156 ng/ml - 10 ng/ml

Reactivity

Mouse

Sample Volume

Concentration(ng/ml)	0	0.156	0.312	0.625	1.25	2.5	5	10
O.D.	0.041	0.073	0.092	0.145	0.301	0.547	1.146	1.854

Description

Mouse Cathepsin E ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ctse in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Ctse; Cathepsin E; EC 3.4.23.34

Immunogen

Expression system for standard: NS0; Immunogen sequence: S60-P397

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Ctse

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	178	785	2784	191	805	2845
Standard deviation	7.30	34.54	141.98	10.51	31.40	173.55
CV(%)	4.1%	4.4%	5.1%	5.5%	3.9%	6.1%

Background

Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	178	191	188	163	180	12.06	6.7%
Sample 2	785	696	814	793	772	37.83	4.9%
Sample 3	2784	2863	2924	2652	2806	154.32	5.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	13034
Gene Name	Ctse
UniProt ID	P70269
Protein Name	Cathepsin E

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001675	EK2128	96 wells/kit	$499		Add to Cart

Mouse Cathepsin E ELISA Kit PicoKine®

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